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KA3897 ACK1 (Phospho-Tyr284) Antibody Quote Quote

Overview

Product NameACK1 (Phospho-Tyr284) Antibody
ApplicationsWB, IHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).
Specificity/SensitivityACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, Non-receptor protein tyrosine kinase Ack, TNK2, kinase ACK1
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesManser E., Nature 363:364-367(1993). Ota T., Nat. Genet. 36:40-45(2004). Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)
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