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Afc-calpain-activity-assay-kit-fluorimetric

Cat. No.	Name	Size	Price	Add Cart
KKS0038	AFC Calpain Activity Assay Kit Fluorimetric	100 assays	$897.26

Overview

Product Name	AFC Calpain Activity Assay Kit Fluorimetric
Kit Summary	Detection method- Fluorescence (Ex/Em 380/500 nm) Applications- The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 500 nm using a fluorometer or a fluorecence plate reader
Features Benefit	Fast and convenient The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately
Kit Components	Extraction Buffer 10X Reaction Buffer Calpain Substrate Ac-LLY-AFC Active Calpain I (Positive Control) Calpain Inhibitor Z-LLY-FMK
Storage Conditions	-20
Shipping Conditions	Gel back
Description	Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2+, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity. The Extraction Buffer provided with the kit specifically extracts cytosolic proteins without contaminations of cell membrane and lysosome proteases. The Extraction Buffer also prevents auto-activation of calpain during the extraction procedure. Thus, the kit detects only activated calpain in cytosol upon treatment of cells with inducers (e.g., chemicals or drugs). The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 380 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

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