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Cat. No. Name Size Price Add Cart
KA4265 Akt (Ab-129) Antibody Quote Quote

Overview

Product NameAkt (Ab-129) Antibody
ApplicationsWB, IHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-SP-G-A).
Specificity/SensitivityAkt (Ab-129) antibody detects endogenous levels of total Akt protein.

Product Information

SourceRabbit
Research AreasPhosphatases/Kinases
Alternative NamesAKT; AKT1 kinase; C-AKT; EC 2.7.11.1; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
WB
Western blot analysis of extracts from HuvEc cells, treated with Serum (30%, 30mins), using Akt (Ab-129) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using Akt (Ab-129) Antibody. The lane on the left is treated with synthesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Akt (Ab-129) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HeLa cells, using Akt (Ab-129) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesJones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991). Matsubara A., Diabetologia 44:910-913(2001). The MGC Project Team; Genome Res. 14:2121-2127(2004).
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KareBayTM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.
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