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KA3998 Akt2 (Phospho-Ser474) Antibody Quote Quote

Overview

Product NameAkt2 (Phospho-Ser474) Antibody
ApplicationsWB, IHC
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).
Specificity/SensitivityAkt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative Nameskinase Akt2; PKB beta; Protein kinase Akt-2; Protein kinase B, beta; RAC-beta serine/threonine protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
IHC
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (phospho- Ser474) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesSun M, et al. (2001) Cancer Res; 61(16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.
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