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KKS0039AMC Calpain Activity Assay Kit *Fluorimetric*100 assays$897.26

Overview

Product NameAMC Calpain Activity Assay Kit *Fluorimetric*
Kit SummaryDetection method- Fluorescence (Ex/Em 354/442 nm)
Applications- The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 442 nm using a fluorometer or a fluorecence plate reader
Features BenefitFast and convenient
The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately
Kit ComponentsExtraction Buffer
10X Reaction Buffer
Calpain Substrate Ac-LLY-AMC
Active Calpain I (Positive Control)
Calpain Inhibitor Z-LLY-FMK
Storage Conditions-20
Shipping ConditionsGel back
DescriptionThe AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 ( (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.

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