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KA3954 AurB (Phospho-Thr232) Antibody Quote Quote

Overview

Product NameAurB (Phospho-Thr232) Antibody
ApplicationsWB, IHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AurB around the phosphorylation site of threonine 232 (R-K-TP-M-C).
Specificity/SensitivityAurB (Phospho-Thr232) antibody detects endogenous levels of AurB only when phosphorylated at threonine 232.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesAIK2, AIM-1, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, EC 2.7.11.1, STK-1, STK12, Serine/threonine protein kinase 12
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
IHC
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using AurB (Phospho-Thr232) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesShindo M., Biochem. Biophys. Res. Commun. 244:285-292(1998). Tatsuka M., Cancer Res. 58:4811-4816(1998). Kimura M., Cytogenet. Cell Genet. 82:147-152(1998).
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