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KKS0037Calpain Activity Assay Kit * Colorimetric*100 assays$897.26

Overview

Product NameCalpain Activity Assay Kit * Colorimetric*
Kit SummaryDetection method- Absorbance (400 or 405 nm )
Applications- The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 500 nm using a fluorometer or a fluorecence plate reader
Features BenefitFast and convenient
The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately
Kit ComponentsExtraction Buffer
10X Reaction Buffer
Calpain Substrate Ac-LLY-pNA
Active Calpain I (Positive Control)
Calpain Inhibitor Z-LLY-FMK
Storage Conditions-20
Shipping ConditionsGel back
DescriptionThe Colorimetric Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a Colorimetric peptide substrate, suc-LLVY-pNA. Upon calpain protease cleavage, suc-LLVY-pNA generates the pNA Colorimetric and can be detection method- Absorbance at 400 or 405 nm. Increase in pNA Colorimetric Absorbance is proportional to calpain activity. The assay can detect both calpain 1 ((μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.

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