Cat. No. Name Size Price Add Cart
KKS0001Caspase-1 Colorimetric Assay Kit25 assays$245.7
Caspase-1 Colorimetric Assay Kit100 assays$609.7

Overview

Product NameCaspase-1 Colorimetric Assay Kit
Kit SummaryDetection method- Absorbance (400- or 405 nm)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis
Features BenefitFast and convenient
Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity
Kit ComponentsCell Lysis Buffer:
2X Reaction Buffer YVAD-pNA (4 mM) pNA(p-Nitroaniline) Colorimetric reference standard
Storage Conditions-20
Shipping ConditionsGel back
DescriptionActivation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

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Our products are used for research, laboratory and further evaluation purposes. They are not for human use.