Cat. No. Name Size Price Add Cart
KKS0008Caspase -2 AFC Fluorometric Assay Kit25 assays$263.9
Caspase -2 AFC Fluorometric Assay Kit100 assays$627.9

Overview

Product NameCaspase -2 AFC Fluorometric Assay Kit
Kit SummaryDetection method- Fluorescence (380 nm excitation filter and 500 nm emission filter)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis
Features BenefitFast and convenient
Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-2 activity
Kit ComponentsCell Lysis Buffer:
2X Reaction Buffer
VDVAD-AFC (1 mM); AFC(7-amido-4-trifluoromethylcoumarin), fluorescence reference standard
Storage Conditions-20
Shipping ConditionsGel back
DescriptionActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (lmax = 380 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 500 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.

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