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KKS0005Caspase - 3/7 AFC Fluorometric Assay Kit0Quote

Overview

Product NameCaspase - 3/7 AFC Fluorometric Assay Kit
Kit SummaryDetection method- Fluorescence (380 nm excitation filter and 500 nm emission filter)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis
Features BenefitFast and convenient
Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-3/7 activity
Kit ComponentsCell Lysis Buffer:
2X Reaction Buffer
DEVD-AFC (1 mM); AFC(7-amido-4-trifluoromethylcoumarin), fluorescence reference standard
Storage Conditions-20
Shipping ConditionsGel back
DescriptionActivation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3/7 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate AC-DEVD-AFC (AFC7-amino-4-trifluoromethyl coumarin). AC-DEVD-AFC emits blue light (lmax = 380 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 500 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/7/CPP32 activity.

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