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Cat. No. Name Size Price Add Cart
KKS0047Cathepsin B Assay Kit *Fluorimetric*100 assays$664.3

Overview

Product NameCathepsin B Assay Kit *Fluorimetric*
Kit SummaryDetection method- Fluorescence (Ex/Em 380/500) and fluorometer
Sample type- Cell and tissue lysates
Species reactivity- Mammalian
Kit size- 100 assays
Applications- Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals
Features BenefitFast and convenient
Cathepsin-B assay is straightforward, and can be adapted to 96-well plate assays
Kit ComponentsCB Cell Lysis Buffer:
CB Reaction Buffer
CB Substrate Ac-RR-AFC (10 mM)
CB Inhibitor (1 mM)
Storage Conditions-20
Shipping ConditionsGel back
DescriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Please click here to fill online order form, and we will make sure to supply you product with detail information. This process usually takes between 24 to 48 hours, depending on products stock avaliability. All inquires and subsequent projects are handled in the strictest confidence and will be backed by a confidentiality agreement if required.
KareBayTM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.
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