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KKS0063Cathepsin D Inhibitor Screening Kit?100 assays$864.5

Overview

Product NameCathepsin D Inhibitor Screening Kit?
Kit SummaryDetection method- Fluorescence
Sample type- Purified Cathepsin D, Recombinant Cathepsin D
Applications- Assay kit to detect putative Cathepsin D inhibitors
Features BenefitFast, robust and convenient
Cathepsin-D inhibitor screening assay is a fast and robust assay to screen potential inhibitors
Positive control inhibitor, Pepstatin A is included in the kit
Kit ComponentsCD Reaction Buffer
CD Substrate (1 mM)
Cathepsin D, human (lyophilized)
Pepstatin A (1 mM in DMSO)
Storage Conditions-20
Shipping ConditionsGel back
DescriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with AFC. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group AFC, which can then easily be measured using a fluorometer or fluorescence plate reader. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).

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