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KA4378 CDC25B (Ab-353) Antibody Quote Quote

Overview

Product NameCDC25B (Ab-353) Antibody
ApplicationsWB, IHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human CDC25B around the phosphorylation site of serine 353 (R-R-SP-V-T).
Specificity/SensitivityCDC25B (Ab-353) antibody detects endogenous levels of total CDC25B protein.

Product Information

SourceRabbit
Research AreasCell Cycle
Alternative NamesCDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Ab-353) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HeLa cells, using CDC25B (Ab-353) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesSanae Uchida, J. Cell Sci., Jun 2004; 117: 3011 - 3020. Christina Karlsson, J. Cell Biol., Aug 1999; 146: 573. Arne Lindqvist, J. Cell Biol., Oct 2005; 171: 35 - 45.
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Our products are used for research, laboratory and further evaluation purposes. They are not for human use.