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KA4129 COT (Ab-290) Antibody Quote Quote

Overview

Product NameCOT (Ab-290) Antibody
ApplicationsWB, IHC
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
Specificity/SensitivityCOT (Ab-290) antibody detects endogenous levels of total COT protein.

Product Information

SourceRabbit
Research AreasPhosphatases/Kinases
Alternative NamesC-COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, EC 2.7.11.25, M3K8, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, TPL-2, TPL2, Tpl2, Tumor progression locus 2, kinase Cot
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:300~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (Ab-290) antibody (#B0064). The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HuvEc cells, using COT (Ab-290) antibody (#B0064). The picture on the right is treated with the synthesized peptide.
ReferencesLawrence P. Kane, Mol. Cell. Biol., Aug 2002; 22: 5962 - 5974. Brenda S. Luciano, J. Biol. Chem., Dec 2004; 279: 52117 - 52123. Matilde Caivano, J. Biol. Chem., Dec 2003; 278: 52124 - 52130. Kenji Sugimoto, J. Clin. Invest., Sep 2004; 114: 857 - 866.
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