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KA4133 Cyclin B1 (Ab-126) Antibody Quote Quote

Overview

Product NameCyclin B1 (Ab-126) Antibody
ApplicationsWB, IHC
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-S-P-S).
Specificity/SensitivityCyclin B1 (Ab-126) antibody detects endogenous levels of total Cyclin B1 protein.

Product Information

SourceRabbit
Research AreasCell Cycle
Alternative NamesCCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Ab-126) antibody (#BOO68). The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Cyclin B1 (Ab-126) antibody (#BOO68). The picture on the right is treated with the synthesized peptide.
ReferencesJing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609. Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280. Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074. Jianke Li, PNAS, Jan 1997; 94: 502.
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