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KKS0078Deubiquitination Assay Kit *Fluorimetric*0Quote

Overview

Product NameDeubiquitination Assay Kit *Fluorimetric*
Kit SummaryDetection method - Fluorescence
Applications - Determine activity of purified Deubiquitination
Features BenefitRapid and convenient
Kit Components440 DUB fluorogenic substrate (Ex/Em=354/442 nm upon cleavage)
Assay buffer
Deubiquitin protease UCH-L3
Inhibitor
Fluorescence reference standard
Storage Conditions-20
Shipping ConditionsGel back
DescriptionAssay Kit provides a convenient assay for the screening of enzyme inhibitors and activators or for continuous assay of deubiquitination activity. A natural substrate, ubiquitin, coupled with the AMC (7-amino-4-methylcoumarin) fluorophore is used in the assay. Upon the cleavage by a deubiquitin protease, this substrate generates the blue fluorophore that can be detected at excitation/emission=354/442 nm.

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