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KA5065 GRB2 Antibody Quote Quote

Overview

Product NameGRB2 Antibody
ApplicationsWB, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized peptide derived from internal of human GRB2.
Specificity/SensitivityGRB2 antibody detects endogenous levels of total GRB2 protein.

Product Information

SourceRabbit
Research AreasIntermediates
Alternative NamesGrowth factor receptor-bound protein 2; Adapter protein GRB2; SH2/SH3 adapter GRB2; Protein AshStorage/Stability: Store at -20??1 year
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)
Application NotesWB: 1:500~1:3000 ELISA: 1:40000
WB
Western blot analysis of extracts from Jurkat cells, using GRB2 antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from JK cells (Lane 2), cos-7 cells (Lane 3) and 293 cells (Lane 4), using GRB2 Antibody. The lane on the left is treated with synthesized peptide.
ReferencesLowenstein E.J., Cell 70:431-442(1992). Matuoka K., Proc. Natl. Acad. Sci. U.S.A. 89:9015-9019(1992). Fath I., Science 264:971-974(1994).
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