Home »
Biochemical Products »
Hdac-activity-assay-kit-amc-fluorimetric

Cat. No.	Name	Size	Price	Add Cart
KKS0042	HDAC Activity Assay Kit *AMC Fluorimetric	100 assays	$707.07

Overview

Product Name	HDAC Activity Assay Kit *AMC Fluorimetric
Kit Summary	Detection method- Fluorescence (Ex/Em 354/442 nm) Sample type- Cell lysate, nuclear extract Species reactivity- Mammalian Applications- Well suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately
Features Benefit	Fast and convenient The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays
Kit Components	HDAC Substrate [Boc-Lys(Ac)-AMC, 4 mM] 10X HDAC Assay Buffer Lysine Developer HDAC Inhibitor (Trichostatin A, 1 mM) HeLa Nuclear Extract (5 mg/ml) Deacetylated Standard [Boc-Lys-AMC, 4 mM]
Storage Conditions	-20
Shipping Conditions	Gel back
Description	Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and induce apoptosis or differentiation in cancer cells. However, screening compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation of the substrate sensitizes the substrate, so that further treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.

Please click here to fill online order form, and we will make sure to supply you product with detail information. This process usually takes between 24 to 48 hours, depending on products stock avaliability. All inquires and subsequent projects are handled in the strictest confidence and will be backed by a confidentiality agreement if required.

KareBay^TM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.

About

KareBay^TM Biochem
Sitemap
Blog

Custom Services

Organic Synthesis
Peptide Synthesis
Antibody Production
Protein Expression
Bioconjugation Service
Immunoassry Service
Molecular Biology Services
Oligonucleotides Synthesis
RNA Synthesis Service
cGMP Peptide Synthesis Services
cGMP Organic Synthesis Services

Biochemical Products

Small Molecule Inhibitors
Bioactive Compounds
Primary Antibodies
Peptides
Amino Acids
Reagents
Fluorescent dyes
Intermediates
PCR

KareBay^TM Biochem, Inc.
11 Deer Park Drive, Suite 102A
Monmouth Junction, NJ 08852

Telephone

: 732-823-1545

Fax

: 732-823-1349

product@karebaybio.com
service@karebaybio.com
support@karebaybio.com