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Hdac-activity-assay-kit-colorimetric

Cat. No.	Name	Size	Price	Add Cart
KKS0040	HDAC Activity Assay Kit * Colorimetric	100 assays	$707.07

Overview

Product Name	HDAC Activity Assay Kit * Colorimetric
Kit Summary	Detection method- Absorbance (400 or 405 nm) Sample type- Cell lysate, nuclear extract Species reactivity- Mammalian Applications- Well suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately
Features Benefit	Fast and convenient The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays
Kit Components	HDAC Substrate [Ac-Lys(Ac)-pNA, 10 mM] 10X HDAC Assay Buffer Lysine Developer HDAC Inhibitor (Trichostatin A, 1 mM) HeLa Nuclear Extract (5 mg/ml) Deacetylated Standard (Ac-Lys-pNA, 10 mM)
Storage Conditions	-20
Shipping Conditions	Gel back
Description	Inhibition of histone deacetylases (HDACs) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells. However, screening HDAC inhibitory compounds has proven to be difficult over the past due to the lack of convenient tools for analyzing HDAC activity. The new Colorimetric HDAC Activity Assay Kit provides a Fast and convenient colorimetric method that eliminates radioactivity, extractions, or chromatography, as used in the traditional assays. The new method requires only two easy steps, both performed on the same microtiter plate. First, the HDAC colorimetric substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract or your own samples). Deacetylation of the substrate sensitizes the substrate, so that, in the second step, treatment with the Lysine Developer produces a chromophore. The chromophore can be easily analyzed using an ELISA plate reader or spectrophotometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.

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Our products are used for research, laboratory and further evaluation purposes. They are not for human use.

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