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KA3962 HMG14 (Phospho-Ser21) Antibody Quote Quote

Overview

Product NameHMG14 (Phospho-Ser21) Antibody
ApplicationsIHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HMG14 around the phosphorylation site of serine 21 (R-R-SP-A-R).
Specificity/SensitivityHMG14 (Phospho-Ser21) antibody detects endogenous levels of HMG14 only when phosphorylated at serine 21.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesHMG14, High-mobility group nucleosome binding domain 1, Nonhistone chromosomal protein HMG-14
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)
Application NotesIHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HMG14 (Phospho-Ser21) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesMihael H. Polymeropoulos. Nucleic Acids Res., Jul 1991; 19: 3753. LJ Arwood. J. Biol. Chem., Jun 1990; 265: 9771 - 9777. Thomas Hildmann. Genome Res., Apr 1999; 9: 360 - 372.
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