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KA4669 IRS-1 (Ab-636/639) Antibody Quote Quote

Overview

Product NameIRS-1 (Ab-636/639) Antibody
ApplicationsWB, IHC, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 or serine 639 (M-S-P-K-S).
Specificity/SensitivityIRS-1 (Ab-636/639) antibody detects endogenous levels of total IRS-1 protein.

Product Information

SourceRabbit
Research AreasIntermediates
Alternative NamesIRS-1, IRS1, Insulin receptor substrate-1
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB:1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000
WB
Western blot analysis of extracts from K562 cells (Lane 2), using IRS-1 (Ab-636) Antibody. The lane on the left is treated with synthesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IRS-1 (Ab-636) Antibody. The picture on the right is treated with the synthesized peptide.
ReferencesOzes ON, et al. (2001) Proc Natl Acad Sci USA; 98(8): 4640-4645. Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76. Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465. Ozes ON, et al. (2001) Proc Natl Acad Sci USA; 98(8): 4640-4645. Szanto I, et al. (2000) Proc Natl Acad Sci USA; 97(5): 2355-2360.
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