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KA4095 MCM3 (Phospho-Thr722) Antibody Quote Quote

Overview

Product NameMCM3 (Phospho-Thr722) Antibody
ApplicationsWB, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human MCM3 around the phosphorylation site of threonine 722.
Specificity/SensitivityMCM3 (Phospho-Thr722) antibody detects endogenous levels of MCM3 only when phosphorylated at threonine 722.

Product Information

SourceRabbit
Research AreasIntermediates
Alternative Namescervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3; MCM3 minichromosome maintenance deficient 3; MGC1157; minichromosome maintenance complex component 3; minichromosome maintenance deficient 3; P1-MCM3; P1.h; p102; replication licensing factor, beta subunit; RLF subunit beta; RLFB
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman; Mouse; Rat
Application NotesWB: 1:500~1:3000 ELISA: 1:10000
WB
Western blot analysis of extracts from K562 cells, A549 cells, COLO cells, HepG2 cells, HuvEc cells and JurKat cells, using MCM3 (Phospho-Thr722) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells (Lane 2), JK cells (Lane 3) and HuvEc cells (Lane 4), using MCM3 (Phospho-Thr722) Antibody. The lane on the left is treated with synthesized peptide.
ReferencesHu B., Nucleic Acids Res. 21:5289-5293(1993). Kubota Y., Cell 81:601-609(1995). Mungall A.J., Nature 425:805-811(2003).
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