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KA4207 MDM2 (Ab-166) Antibody Quote Quote

Overview

Product NameMDM2 (Ab-166) Antibody
ApplicationsWB, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T).
Specificity/SensitivityMDM2 (Ab-166) antibody detects endogenous levels of total MDM2 protein.

Product Information

SourceRabbit
Research AreasCancer/Apoptosis
Alternative NamesDouble minute 2 protein, EC 6.3.2.-, Oncoprotein Mdm2, Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse
Application NotesWB: 1:500~1:3000 IF: 1:100~1:500 ELISA: 1:1000
IF
Immunofluorescence analysis of NIH/3T3 cells, using MDM2 (Ab-166) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesM Zhou, Blood, Mar 1995; 85: 1608 - 1614. Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030. M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228. Jordan S. Fridman, Cancer Res., Sep 2003; 63: 5703 - 5706.
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