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KA2982 PYK2 (Phospho-Tyr579) Antibody Quote Quote

Overview

Product NamePYK2 (Phospho-Tyr579) Antibody
ApplicationsWB, IHC, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 579 (E-D-YP-Y-K).
Specificity/SensitivityPYK2 (Phospho-Tyr579) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesCADTK; CAK beta; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; EC 2.7.10.2; FADK 2; FAK2; Focal adhesion kinase 2; kinase Pyk2; Proline-rich tyrosine kinase 2; Protein tyrosine kinase 2 beta; PTK2B; RAFTK; Related adhesion focal tyrosine kinase
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000
WB
Western blot analysis of extracts from 3T3 cells, using PYK2 (Phospho-Tyr579) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with synthesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesLev S., Nature 376:737-745(1995). Herzog H., Genomics 32:484-486(1996). Sasaki H., J. Biol. Chem. 270:21206-21219(1995).
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