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KA3086 Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody Quote Quote

Overview

Product NameRho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody
ApplicationsWB, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of serine 885 (R-R-SP-L-P).
Specificity/SensitivityRho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody detects endogenous levels of Rho/Rac Guanine Nucleotide Exchange Factor 2 only when phosphorylated at serine 885

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesGEF-H1, GEFH1, Guanine nucleotide exchange factor GEF-H1, proliferating cell nucleolar antigen p40, rho/rac guanine nucleotide exchange factor 2
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse
Application NotesWB: 1:500~1:3000 ELISA: 1:5000
WB
Western blot analysis of extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin(40nM, 30mins), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells (Lane 2), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the left is treated with synthesized peptide.
ReferencesRen Y., J. Biol. Chem. 273:34954-34960(1998). Krendel M., Nat. Cell Biol. 4:294-301(2002). Ishikawa K., DNA Res. 5:169-176(1998)
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