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KA3250 SGK (Ab-422) Antibody Quote Quote

Overview

Product NameSGK (Ab-422) Antibody
ApplicationsWB, IHC, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human SGK around the phosphorylation site of serine 422 (G-F-SP-Y-A).
Specificity/SensitivitySGK (Ab-422) antibody detects endogenous levels of total SGK protein.

Product Information

SourceRabbit
Research AreasPhosphatases/Kinases
Alternative NamesSGK1, SERUM/GLUCOCORTICOID-REGULATED KINASE
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:5000
WB
Western blot analysis of extracts from Jurkat cells, using SGK (Ab-422) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from colo cells (Lane 2) and K562 cells (Lane 3), using SGK (Ab-422) Antibody. The lane on the left is treated with synthesized peptide.
IHC
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SGK (Ab-422) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesNicola Perrotti, J. Biol. Chem., Mar 2001; 276: 9406 - 9412. Yun L. Ma, Learn. Mem., Mar 2006; 13: 114 - 118. Ge-Xin Wang, Am J Physiol Heart Circ Physiol, Aug 2004; 287: H533 - H544.
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Our products are used for research, laboratory and further evaluation purposes. They are not for human use.