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KA3469 TAK1 (Phospho-Thr184) Antibody Quote Quote

Overview

Product NameTAK1 (Phospho-Thr184) Antibody
ApplicationsWB, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human TAK1 around the phosphorylation site of threonine 184 (I-Q-TP-H-M).
Specificity/SensitivityTAK1 (Phospho-Thr184) antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesEC 2.7.11.25, M3K7, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, TGF-beta- activated kinase 1, Transforming growth factor-beta-activated kinase 1, kinase TAK1
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:3000 ELISA: 1:1000
WB
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells (Lane 2), using TAK1 (Phospho-Thr184) Antibody. The lane on the left is treated with synthesized peptide.
ReferencesSakurai H., Biochem. Biophys. Res. Commun. 243:545-549(1998). Mungall A.J., Nature 425:805-811(2003). The MGC Project Team; Genome Res. 14:2121-2127(2004)
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