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KA4251 TNNI3 (Ab-22/23) Antibody Quote Quote

Overview

Product NameTNNI3 (Ab-22/23) Antibody
ApplicationsWB, IHC, IF, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human TNNI3 around the phosphorylation site of serine 22 (R-R-SP-SP-A-N).
Specificity/SensitivityTNNI3 (Phospho-Ser22+Ser23) antibody detects endogenous levels of total TNNI3 protein.

Product Information

SourceRabbit
Research AreasCytoskeletal
Alternative NamesTNNC1, troponin I, cardiac muscle
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman, Mouse, Rat
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
IHC
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Phospho-Ser22+Ser23) antibody. The picture on the right is treated with the synthesized peptide.
IF
Immunofluorescence analysis of HepG2 cells, using TNNI3 (Phospho-Ser22+Ser23) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesRaffaella Di Lisi, J. Biol. Chem., Sep 1998; 273: 25371. Emile Missov, Circulation, Nov 1997; 96: 2953 - 2958. Brandon J. Biesiadecki, J. Biol. Chem., Apr 2004; 279: 13825 ??13832.
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