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KA3606 TOB1 (Phospho-Ser164) Antibody Quote Quote

Overview

Product NameTOB1 (Phospho-Ser164) Antibody
ApplicationsWB, IHC, ELISA
ConcentrationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human TOB1 around the phosphorylation site of serine 164 (A-V-SP-P-T).
Specificity/SensitivityTOB1 (Phospho-Ser164) antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

Product Information

SourceRabbit
Research AreasPhospho-specific
Alternative NamesTOB; Transducer of erbB-2 1
Storage/StabilityStore at -20/1 year
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)
Application NotesWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:5000
WB
Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using TOB1 (Phospho-Ser164) antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with systhesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody. The picture on the right is treated with the synthesized peptide.
ReferencesMatsuda S., Oncogene 12:705-713(1996). Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
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