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KKS0083Trypsin Activity Colorimetric Assay Kit100 assays$591.5

Overview

Product NameTrypsin Activity Colorimetric Assay Kit
Kit SummaryDetection method- Absorbance (405 nm)
Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc
Features BenefitFast and convenient
Kit ComponentsTrypsin Assay Buffer
Trypsin Substrate (in DMSO)
Positive Control (~2U, lyophilized)
p-NA Standard (2 mM)
Trypsin Inhibitor (TLCK, 20 mM)
Chymotrypsin Inhibitor (TPCK,10 mM)
Storage Conditions-20
Shipping ConditionsGel back
DescriptionTrypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.

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