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Cat. No. Name Size Price Add Cart
KP0131 360 MMP FRET Substrate I 1 mg $95

Product Information

Product Name360 MMP FRET Substrate I
Purity% Peak Area By HPLC ≥ 95%
Molecular Weight 977.1
Storage-20°C
Sequence
(One-Letter Code)		Dnp-PLGLWAr-NH2
Sequence
(Three-Letter Code)		Dnp - Pro - Leu - Gly - Leu - Trp - Ala - D - Arg - NH2
DescriptionA generic MMP fluorescent substrate, originally prepared as a substrate for fibroblast collagenase. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P'1 bond, giving rise to a continuously recording fluorescence assay. The hydrolysis rates of the FRET peptide rival or exceed that of its natural protein substrates. This substrate also demonstrates that its water solubility in the typical MMP assay buffer exceeds the Km value for the enzyme, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.
ReferencesRef: Santala, A. et al. FEBS Lett. 461, 153 (1999); Bickett, DM. et al. Anal. Biochem. 212, 58 (1993); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991); Stack, M. et al. J. Biol. Chem. 264, 4277 (1989).
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KareBayTM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.
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