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Cat. No. Name Size Price Add Cart
KP0136 360 MMP FRET Substrate IV 1 mg $68

Product Information

Product Name360 MMP FRET Substrate IV
Purity% Peak Area By HPLC ≥ 95%
Molecular Weight 1152.3
Storage-20°C
Sequence
(One-Letter Code)		Dnp-PYAYWMR
Sequence
(Three-Letter Code)		Dnp - Pro - Tyr - Ala - Tyr - Trp - Met - Arg - OH
DescriptionThis substrate has a fluorescent Trp residue in subsite P'2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P'1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P'1 and P'3 is optimized for MMP-3, while Arg is placed in P'4 to enhance solubility. The substrate hydrolysis rate rivals or exceeds those of the corresponding natural protein substrates or other synthetic peptides. The solubility of this substrate in assay buffer exceeds the Km value for each reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.
ReferencesRef: Finch-Arietta, M. et al. Agents Actions 39, C189 (1993); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
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KareBayTM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.
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