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Cat. No. Name Size Price Add Cart
KP1201 MMP Colorimetric Substrate I 1 mg $116

Product Information

Product NameMMP Colorimetric Substrate I
Purity% Peak Area By HPLC ≥ 95%
Molecular Weight 655.9
Storage-20°C
Sequence
(One-Letter Code)		Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
Sequence
(Three-Letter Code)		Ac - Pro - Leu - Gly - SCH[CH2CH(CH3)2] - CO - Leu - Gly - OC2H5
DescriptionAn exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4'dithiodipyridine or Ellman's Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.
ReferencesRef: Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985); Weingarten, H. et al. Biochem. 24, 6730 (1985).
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KareBayTM provides scientists and clinicians with a wide range of biotechnological products and science lab supplies for chemical research and analyzing life processes. KareBay's extensive capabilities include commercializing reagents and kits, manufacturing biotech products and providing contract research services to organizations worldwide. Our many global labs, offices, and business partners enable KareBay to extend its products and services to its customer base around the world.

Our products are used for research, laboratory and further evaluation purposes. They are not for human use.
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