Custom Monoclonal Antibody Production
A monoclonal antibody of high purity, titer and strong specificity could avoid serological cross-reaction of different cells and microorganisms between species or strains, and greatly improve diagnostic specificity and sensitivity. Such reagents also play a critical role in the study of cell surface markers, as soluble antigens for purification and in the investigation of an antibody’s structure and function. KareBayTM Biochem provides custom monoclonal antibody production services, which are operated by a professional staff with many years of experience. Our modern production technology is proven to generate reliable products and we will work with you to provide the best antibody production solution possible according to your specific requirements.
At every production step the antibodies are strictly screened and validated in order to ensure the products meet customer expectations. We implement our professional antigen design software as an integral component of our antibody production process and our antibody success rate is typically greater than 98%.
Monoclonal Antibody Production Protocol:
|
Time (Days)
|
Phase
|
Description
|
|
1
|
Primary immune | Immunize 5 BALB/c mice per antigen, Antigen+FCA |
|
14
|
Secondary immune | Antigen+ water-soluble adjuvant |
|
21
|
The third immune | Antigen+ water-soluble adjuvant |
|
28
|
Serum titer detection | Serum titer detection by indirect ELISA method, titer>1:20,000 If titer>10,000, continue the next immune If titer <10,000, end the contract |
|
30
|
Strengthen the immune | Antigen 3 days later, blood collection (serum as positive control), take the spleen |
|
34
|
Cell fusion | Fusion of SP2/0 myeloma cell and a mouse spleen cell with high ELISA titer |
|
44-56
|
Hybridoma cells screened by HAT | Screen a positive cell clone by ELISA method |
|
60-70
|
Delivery of cell supernatant | ELISA positive cell supernatant (2ml) will be delivered to customer for detection. Please reply us in a week |
| Cell preservation | Choose a cell strain for the following ascites production | |
| Ascites production and purification | Vaccinate cells for ascites production and purification (one strain) | |
|
|
Delivery | 2 strains of hybridoma cells, T-25 cell (a cell culture flask) 1 strain of cell protein A/G purified antibody 2mg Experiment reports Antibody acceptance standard: immunogen detection of antibody titer >1:50,000 |
Services Specifications:
|
Cat.No.
|
Services
|
Description
|
Timeline
|
Price
|
|
KB050
|
Standard mAb Service | All the positive hybridoma cells, protein A purification of one assigned cell strain (immunogen recognition by WB approach) |
18-20wks
|
Quote
|
|
KB051
|
Ascites Production | Mouse ascites 10ml |
3-4wks
|
Quote
|
|
KB052
|
Antibody Recognition Epitopes Competitive Analysis | Customer supplies mAb ascites |
3-4wks
|
Quote
|
|
KB053
|
Peptide synthesis + Standard mAb Service | Customer supplies the peptide sequence (<15aa) |
20-23wks
|
Quote
|
|
KB202
|
Phosphorylation/Methylation/Acetylation Antibody Production | Modified peptide synthesis |
22-25wks
|
Quote
|
|
KB204
|
WB Guarantee mAb production | WB positive antibody production |
22-25wks
|
Quote
|
|
KB244
|
IP Guarantee mAb Production | IP positive antibody production |
22-25wks
|
Quote
|
|
KB245
|
IHC Guarantee mAb Production | IHC positive antibody production |
22-25wks
|
Quote
|
|
KB246
|
FC Guarantee mAb Production | FC positive antibody production |
22-25wks
|
Quote
|
|
KB247
|
Fast Antibody Production Package | Fast antibody production |
22-25wks
|
Quote
|
|
KB054
|
Protein Expression + Protein Purification+mAb Standard Service | Customer supplies the expression plasmid |
20-23wks
|
Quote
|
|
KB055
|
Vector Construction + Protein Expression + Protein Purification+ mAb Standard Service | Customer supplies the PCR template |
22-26wks
|
Quote
|
|
KB056
|
Gene Synthesis + Vector Construction + Protein Expression + Protein Purification + mAb Standard Service | Customer supplies the gene sequence |
25-28wks
|
Quote
|
|
KB057
|
Sandwich ELISA Pairing mAb Production | Select sandwich ELISA pairing mAb on the basis of mAb standard service |
28-32wks
|
Quote
|
|
KB118
|
The Establishment of Indirect Competitive ELISA Detection Method | Conditions optimization and antigenic competition epitopes mAb selection |
4-8wks
|
Quote
|
How to get started Please contact us with your requests at service@karebaybio.com and we will reply with a detailed quote as soon as possible. This process usually takes between 24 and 48 hours and the quote will include an estimated price as well as the time required to complete the project. All inquiries and subsequent projects are handled in strict confidence and will be backed by a confidentiality agreement if desired.
