Primers and probes for 2019-nCoV Coronavirus:
Karebay can provide the primer and probe sequences approved by the CDC and the WHO for rapid identification of the virus. Quality and purity of all the primers from KareBay are guaranteed with strict quality control system.
The primers and probes are available for research use only.
Please do not hesitate to contact us:service@karebaybio.com
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CDC Approved Sequences |
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Name |
Description |
Sequence (5′ – 3′) |
Label |
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2019-nCoV_N1-F |
2019-nCoV_N1 Forward Primer |
GACCCCAAAATCAGCGAAAT |
None |
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2019-nCoV_N1-R |
2019-nCoV_N1 Reverse Primer |
TCTGGTTACTGCCAGTTGAATCTG |
None |
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2019-nCoV_N1-P |
2019-nCoV_N1 Probe |
ACCCCGCATTACGTTTGGTGGACC |
FAM BHQ1 |
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2019-nCoV_N2-F |
2019-nCoV_N2 Forward Primer |
TTACAAACATTGGCCGCAAA |
None |
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2019-nCoV_N2-R |
2019-nCoV_N2 Reverse Primer |
GCGCGACATTCCGAAGAA |
None |
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2019-nCoV_N2-P |
2019-nCoV_N2 Probe |
ACAATTTGCCCCCAGCGCTTCAG |
FAM BHQ1 |
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2019-nCoV_N3-F |
2019-nCoV_N3 Forward Primer |
GGGAGCCTTGAATACACCAAAA |
None |
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2019-nCoV_N3-R |
2019-nCoV_N3 Reverse Primer |
TGTAGCACGATTGCAGCATTG |
None |
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2019-nCoV_N3-P |
2019-nCoV_N3 Probe |
AYCACATTGGCACCCGCAATCCTG |
FAM BHQ1 |
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RP-F |
RNAse P Forward Primer |
AGATTTGGACCTGCGAGCG |
None |
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RP-R |
RNAse P Reverse Primer |
GAGCGGCTGTCTCCACAAGT |
None |
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RP-P |
RNAse P Probe |
TTCTGACCTGAAGGCTCTGCGCG |
FAM BHQ1 |
WHO Approved Sequences
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Name |
Description |
Sequence (5′ – 3′) |
Label |
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RdRP_SARSr-F2 |
RdRP gene |
GTGARATGGTCATGTGTGGCGG |
None |
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RdRP_SARSr-R1 |
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CARATGTTAAASACACTATTAGCATA |
None |
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RdRP_SARSr-P2 |
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CAGGTGGAACCTCATCAGGAGATGC |
FAM BHQ1 |
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RdRP_SARSr-P1 |
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CCAGGTGGWACRTCATCMGGTGATGC |
FAM BHQ1 |
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E_Sarbeco_F1 |
E gene |
ACAGGTACGTTAATAGTTAATAGCGT |
None |
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E_Sarbeco_R2 |
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ATATTGCAGCAGTACGCACACA |
None |
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E_Sarbeco_P1 |
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ACACTAGCCATCCTTACTGCGCTTCG |
FAM BHQ1 |
Chinese CDC
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Name |
Description |
Sequence (5′ – 3′) |
Label |
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2019-nCoV-OFP |
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CCCTGTGGGTTTTACACTTAA |
None |
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2019-nCoV-ORP |
ORF1ab |
ACGATTGTGCATCAGCTGA |
None |
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2019-nCoV-OP |
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CCGTCTGCGGTATGTGGAAAGGTTATGG |
FAM BHQ1 |
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2019-nCoV-NFP |
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GGGGAACTTCTCCTGCTAGAAT |
None |
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2019-nCoV-NRP |
Nucleoprotein-protein N |
CAGACATTTTGCTCTCAAGCTG |
None |
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2019-nCoV-NP |
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TTGCTGCTGCTTGACAGATT |
FAM BHQ1 |
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