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Cat. No. Name Size Price Add Cart
KI1255Cyclophosphamide50 mg$154

Chemical Characteristic

Product NameCyclophosphamide
SynonymsCytoxan, Clafen, Endoxan, Neosar, Procytox, Revimmune, Cytophosphane
CAS No.50-18-0
Molecular Weight 261.09
FormulaC7H15Cl2N2O2P
Chemical Name1-(Bis(2-chloroethyl)amino)-1-oxo-2-aza-5-oxaphosphoridine; 2-(Di(2-chloroethyl)amino)-1-oxa-3-aza-2-phosphacyclohexane 2-oxide
SmilesO1P(=O)(NCCC1)N(CCCl)CCCl
Chemical Structure

Biological activities

Cyclophosphamide is a cytotoxic agent in the treatment of certain neoplasms (include malignant lymphomas, leukemias, multiple myeloma, neuroblastoma, retinoblastoma, sarcomas and carcinomas of the ovary, testis, breast, and lung). Cyclophosphamide markedly boosts the cytotoxic activity of lymphocytes against cells from cultured B-leukemia 3163. Cyclophosphamide is effective in augmenting NK cell activity at concentrations ranging from 7.5 to 60 µg/mL. [1] Cyclophosphamide inhibits HEPM cell with an IC50 of 0.3 µM. [2] Cyclophosphamide causes concentration-related increases in sister chromatid exchange (SCE) up to 3 times control frequencies. Cyclophosphamide induces consistently 2.5 to 3.7 more SCEs in B-cells than in T-cells. In vivo, exposure to cyclophosphamide results in significant, dose-related increases in the SCE frequencies of mouse peripheral blood T- and B-lymphocytes. [3] Cyclophosphamide has been recognized as a prodrug which undergoes a complex metabolism in vivo. And 4-hydroxycyclophosphamide is the initial metabolite formed after administration of cyclophosphamide. 4-Hydroperoxycyclophosphamide inhibits MCF-7 and EMT6 cells with IC90 of 33 and 40 µM, respectively. [4]

Protocols

Cyclophosphamide is dissolved in distilled water and diluted to a concentration of 1 mg/mL with MEM before use. [1]

References

[1] Sharma B, et al. Augmentation of human natural killer cell activity by cyclophosphamide in vitro. Cancer Res. 1984, 44(8): 3258-3261.
[2] Pratt RM, et al. In vitro screening assay for teratogens using growth inhibition of human embryonic cells. Proc Natl Acad Sci. 1985, 82(17): 5791-5714.
[3] Wilmer JL, et al. Sister chromatid exchange induction in mouse B- and T-lymphocytes exposed to cyclophosphamide in vitro and in vivo. Cancer Res. 1984, 44(3): 880-884.
[4] Teicher BA, et al. Combination of N, N', N"-triethylenethiophosphoramide and cyclophosphamide in vitro and in vivo. Cancer Res. 1988, 48(1): 94-100.

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